Supplementary Figures S3A, B ). The percentages and cell numbers of each cell population are shown. (D) GC B cells (percentages, ** p = 0.0078, * p = 0.0132; cell numbers, p = 0.0651, * p = 0.0173) and IgG1 + GC cells (percentages, ** p = 0.0070; cell numbers, ** p = 0.0098). (E) Plasma cells (percentages, ** p = 0.0027, * p = 0.0293; cell numbers, *** p = 0.0003, * p = 0.0212) and IgG1 + plasma cells (percentages, * p = 0.0119; cell numbers, * p = 0.0251). (F) Tfh cells (percentages, * p = 0.0332, * p = 0.0202; cell numbers, ** p = 0.0011, p = 0.0713). (G) Representative confocal images of spleen sections visualized for CD4 (green) and PNA (red). Graphs show the percentages of CD4 + areas localized within the PNA + GC areas measured in CD4 Cre/+ Il9ra fl/fl and control CD4 +/+ Il9ra fl/fl mice 7 days after immunization with SRBCs. Imaging data were analyzed using ImageJ software. * p = 0.0162. Data represent the mean ± SD of 7-11 mice per group. Statistical significance was analyzed using the unpaired t -test (B–G) . Similar results were obtained in two-four independent experiments. " width="100%" height="100%">
Journal: Frontiers in Immunology
Article Title: Interleukin 9 mediates T follicular helper cell activation to promote antibody responses
doi: 10.3389/fimmu.2024.1441407
Figure Lengend Snippet: IL-9 assists Tfh cells in promoting GC formation. (A) Experimental protocol for the immunization of CD4 Cre/+ Il9ra fl/fl and control CD4 +/+ Il9ra fl/fl mice. Sera were collected from the tail vein. (B) Serum levels of SRBC-specific IgM and IgG. * p = 0.0476. (C) Representative confocal images of spleen sections, with B cells (CD19 + ) in white and GCs (PNA + ) in red. Graphs show individual values of PNA + GC areas in a tissue section, calculated using Zen software. * p = 0.0491. (D–F) Cell populations in the spleen ( Supplementary Figures S3A, B ). The percentages and cell numbers of each cell population are shown. (D) GC B cells (percentages, ** p = 0.0078, * p = 0.0132; cell numbers, p = 0.0651, * p = 0.0173) and IgG1 + GC cells (percentages, ** p = 0.0070; cell numbers, ** p = 0.0098). (E) Plasma cells (percentages, ** p = 0.0027, * p = 0.0293; cell numbers, *** p = 0.0003, * p = 0.0212) and IgG1 + plasma cells (percentages, * p = 0.0119; cell numbers, * p = 0.0251). (F) Tfh cells (percentages, * p = 0.0332, * p = 0.0202; cell numbers, ** p = 0.0011, p = 0.0713). (G) Representative confocal images of spleen sections visualized for CD4 (green) and PNA (red). Graphs show the percentages of CD4 + areas localized within the PNA + GC areas measured in CD4 Cre/+ Il9ra fl/fl and control CD4 +/+ Il9ra fl/fl mice 7 days after immunization with SRBCs. Imaging data were analyzed using ImageJ software. * p = 0.0162. Data represent the mean ± SD of 7-11 mice per group. Statistical significance was analyzed using the unpaired t -test (B–G) . Similar results were obtained in two-four independent experiments.
Article Snippet: CD19 + B cells and CD3 + T cells were removed using the MagniSort mouse CD19 and CD3 positive selection kits (Thermo Fisher Scientific).
Techniques: Control, Software, Clinical Proteomics, Imaging
